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KS3 Science · Year 8Chapter 2 · Organs, digestion and enzymes
Year 8 · Chapter 2

Organs, digestion and enzymes

By the end of this chapter you will be able to

  • Put cells, tissues, organs and organ systems in order, and name examples
  • Name the organs of the digestive system and say what each one does
  • Explain mechanical and chemical digestion, and name the enzymes that digest starch, protein and fat
  • Use the lock-and-key model to explain how enzymes work, and read a temperature or pH graph
  • Explain osmosis and active transport, and say when each one is needed

You should already know

  • Cells, tissues and organs (Year 7 Chapter 2 and 3)
  • Diffusion and particles (Year 7 Chapter 5)

Where this leads

  • Respiration, which supplies the energy for active transport (Chapter 3)

National Curriculum for England, Key Stage 3 science, Year 8. Content: 8B1 cells are organised into tissues, organs and organ systems (briefly), 8B2 the human digestive system: its organs, mechanical and chemical digestion, enzymes as biological catalysts with lock-and-key action, and the effects of temperature and pH, 8B3 movement of substances into and out of cells: diffusion revisited, osmosis, and active transport.

Exercise P  Do you remember?

1
FWhat is the name of the smallest unit of a living organism? Write the word.
2
FWhat is the main sugar that cells use for energy? Write the word.
3
FWhat is 3 quarters of 20? Write the number.
4
FWhat is the word for the movement of particles from a high concentration to a low concentration? Write the word.
5
FIs a solid that dissolves in water soluble or insoluble? Write the word.
2.1

Cells, tissues, organs and organ systems

Year 8
8B1: cells are organised into tissues, organs and organ systems
Video lesson: Cells, tissues, organs and organ systemsComing soon
Story. Ali is looking at a slide of the lining of his stomach under a microscope. “Is this one thing, or many?” he asks. His teacher says, “It is many cells of the same kind, working together. We call that a tissue, and the stomach is an organ made of several tissues.”

Cells of the same type that work together form a tissue. Different tissues that work together for one job form an organ. Organs that work together for a body job form an organ system. The digestive system is one example.

The stomach wall is an organ made of several tissues. Its muscle tissue churns the food. Its glandular tissue makes digestive juices.

Key ideas

  • A tissue is a group of similar cells working together.
  • An organ is made of different tissues working together for one job.
  • An organ system is a group of organs working together.
  • Order from smallest to largest: cell, tissue, organ, organ system.
Worked example 2.1.1
What is a group of similar cells working together called? Write the word.

Method. Recall the level of organisation.

  1. Think. Similar cells, working together, form the next level up from a cell.
    similar cells
  2. Name it. That level is a tissue.
    tissue
  3. Write the answer.
    tissue

Check. Muscle is one example of a tissue. ✓

Worked example 2.1.2
Name the organ system that includes the stomach, the liver and the mouth. Write the words.

Method. Find the system for digestion.

  1. Look. The stomach, liver and mouth all take part in breaking down food.
    breaking down food
  2. Name it. Organs that work together to digest food form the digestive system.
    digestive system
  3. Write the answer.
    digestive system

Check. These are all organs of the digestive system. ✓

Worked example 2.1.3
Which tissue in the stomach wall churns the food? Write the words.

Method. Think about what churns.

  1. Think. Churning needs a tissue that can contract and squeeze.
    contract
  2. Name it. The muscle tissue of the stomach wall churns the food.
    muscle tissue
  3. Write the answer.
    muscle tissue

Check. The stomach wall has several tissues. ✓

Watch out

Watch out: a single cell is not a tissue. A tissue needs many similar cells working together.

Exam tip

Exam tip. Name the level in order: cell, tissue, organ, organ system, organism.

Try it (with your teacher, or a grown-up at home). Look at a labelled picture of a leaf or a cheek cell, and name each level: cell, tissue, organ. Say which level is the largest. Do not cut any living tissue yourself.

Exercise 2A  Cells, tissues, organs and organ systems

1
FWhat is a group of similar cells working together called? Write the word.
2
FWhat is a structure made of different tissues working together for one job called? Write the word.
3
FWhat is a group of organs working together for a body job called? Write the words.
4
FWhich is larger: a tissue or an organ? Write the word.
5
FName the organ system that includes the mouth, the stomach and the liver. Write the words.
6
FWhich organ makes bile? Write the word.
7
FWhich tissue in the stomach wall contracts to churn food? Write the words.
8
FIs the heart an organ? Write yes or no.
9
FIs a single muscle cell a tissue? Write yes or no.
10
FName the organ system that carries blood around the body. Write the words.
11
FPut these in order from smallest to largest: organ, cell, tissue. Write the three words.
12
PSAli says a stomach is a tissue because it is made of muscle. Is Ali right? Write yes or no.
13
PSWorking scientifically: a student names the levels of the body in order from simplest to most complex. Write the three words for the first three levels.
14
RExplain why an organ can carry out a job that one tissue cannot do alone. Write one sentence.
2.2

The digestive system

Year 8
8B2: the organs of the human digestive system, and mechanical and chemical digestion
Video lesson: The digestive systemComing soon
Story. Ruby eats a cheese sandwich at lunch. “How does the bread turn into something my body can use?” she asks. Her teacher says, “Food is broken down, first by chewing and churning, then by enzymes, before the small pieces can pass into the blood.”

Digestion is the breaking down of large, insoluble food molecules into small, soluble ones. It has two kinds:

mouthoesophagusstomachsmall intestinelarge intestineliverpancreasFood passes from the mouth down to the large intestine.
The digestive system: food moves down the tube from the mouth to the large intestine, with the liver and pancreas beside it.

The table gives the main job of each organ.

OrganMain job
mouthteeth chew food; saliva starts starch digestion
oesophagusa muscular tube that moves food to the stomach
stomachchurns food; makes acid and the enzyme pepsin
livermakes bile, which helps digest fat
pancreasmakes amylase, protease and lipase
small intestinecompletes digestion and absorbs most food
large intestineabsorbs water from the remaining food

Key ideas

  • Digestion breaks large insoluble molecules into small soluble ones.
  • Mechanical digestion breaks food into smaller pieces; chemical digestion uses enzymes.
  • Most absorption happens in the small intestine.
  • The large intestine absorbs water from the remaining food.
Worked example 2.2.1
Which organ absorbs most of the digested food into the blood? Write the words.

Method. Find the organ that absorbs food.

  1. Look. Most absorption happens in the long, folded lining of this organ.
    folded lining
  2. Name it. The small intestine absorbs most of the digested food.
    small intestine
  3. Write the answer.
    small intestine

Check. The large intestine absorbs water. ✓

Worked example 2.2.2
Which type of digestion is chewing with teeth? Write the word.

Method. Decide whether the chemicals change.

  1. Think. Chewing makes the food smaller, but the chemicals stay the same.
    stay the same
  2. Name it. Breaking food into smaller pieces without changing it chemically is mechanical digestion.
    mechanical
  3. Write the answer.
    mechanical

Check. Enzymes make chemical digestion. ✓

Worked example 2.2.3
Which organ makes hydrochloric acid and the enzyme pepsin? Write the word.

Method. Recall the organ that makes acid.

  1. Recall. The stomach makes hydrochloric acid and pepsin.
    stomach
  2. Name it. So the organ is the stomach.
    stomach
  3. Write the answer.
    stomach

Check. The stomach contents are acidic. ✓

Watch out

Watch out: the pancreas and the liver are not part of the tube of food. They make substances that are poured into it.

Exam tip

Exam tip. Name the organ and its job. For example, “the stomach churns food and makes acid”.

Try it (with your teacher, or a grown-up at home). Look at the digestive system picture and trace the path of a piece of bread from the mouth to the large intestine. At each organ say whether digestion is mechanical, chemical, or absorption.

Exercise 2B  The digestive system

1
FWhich organ squeezes and churns the food? Write the word.
2
FWhich organ stores the bile made by the liver? Write the words.
3
FWhich organ absorbs water from the remaining food? Write the words.
4
FWhich organ makes the enzymes amylase, protease and lipase? Write the word.
5
FWhat does mechanical digestion do to food? Write one word: smaller, bigger or weaker.
6
FWhich enzyme breaks down starch? Write the word.
7
FWhich enzyme breaks down proteins into amino acids? Write the word.
8
FWhich enzyme breaks down fats into fatty acids and glycerol? Write the word.
9
PSRuby says chewing is chemical digestion. Is Ruby right? Write yes or no.
10
PSThe stomach contents are very acidic. Is the stomach acidic or alkaline? Write the word.
11
PSWorking scientifically: a pupil draws the digestive system and labels four organs. Name the organ where food is stored before it moves into the small intestine. Write the word.
12
RExplain why digestion is needed before food can be absorbed into the blood. Write one sentence.
2.3

Enzymes as biological catalysts

Year 8
8B2: enzymes are biological catalysts; lock-and-key action at the active site
Video lesson: Enzymes as biological catalystsComing soon
Story. Sam chews a plain cracker for a long time and notices it starts to taste sweet. “Nothing has been added to it,” he says. His teacher says, “Saliva contains an enzyme that breaks starch into sugars. Enzymes do this job very quickly.”

An enzyme is a protein that acts as a biological catalyst. A catalyst speeds up a reaction and is not used up. The molecule an enzyme acts on is the substrate. The enzyme has an active site with a specific shape, and the substrate fits into it. This is the lock-and-key model. The substrate is changed into products, and the enzyme is released unchanged.

enzymeactive sitesubstrateproducts
A substrate fits the active site of an enzyme, which breaks it into products.

The table gives the three main digestive enzymes.

EnzymeSubstrateProducts
amylasestarchsugars
proteaseproteinsamino acids
lipasefatsfatty acids and glycerol

Key ideas

  • Enzymes are proteins that act as biological catalysts.
  • The substrate fits the active site of one specific enzyme.
  • Enzymes are not used up, so a small amount can break down a lot of substrate.
  • Each enzyme changes its own substrate into specific products.
Worked example 2.3.1
What is the molecule that an enzyme acts on called? Write the word.

Method. Recall the definition.

  1. Recall. The molecule an enzyme acts on is the substrate.
    substrate
  2. Check. The substrate is changed into products by the enzyme.
    changed into products
  3. Decide. So the name is substrate.
    substrate
  4. Write the answer.
    substrate

Check. The products are made from the substrate. ✓

Worked example 2.3.2
What is the part of an enzyme where the substrate fits? Write the words.

Method. Recall the part of the enzyme.

  1. Recall. The substrate fits into the active site.
    active site
  2. Check. The active site has a specific shape that matches the substrate.
    specific shape
  3. Decide. So the part is the active site.
    active site
  4. Write the answer.
    active site

Check. The active site has a specific shape. ✓

Worked example 2.3.3
Is an enzyme a protein or a sugar? Write the word.

Method. Recall what enzymes are made of.

  1. Recall. Enzymes are proteins.
    protein
  2. Decide. So an enzyme is a protein, not a sugar.
    protein
  3. Write the answer.
    protein

Check. Protein molecules have specific shapes. ✓

Watch out

Watch out: an enzyme is not used up in the reaction. It can be used again, and it is released unchanged.

Exam tip

Exam tip. Describe the lock-and-key model in one sentence: the substrate fits the active site of one specific enzyme.

Try it (with your teacher, or a grown-up at home). With a grown-up, chew a plain cracker slowly and notice when it starts to taste sweet. Say which enzyme you think is at work, and which substrate it acts on. Do not add any substance to your mouth or to anyone else’s.

Exercise 2C  Enzymes as biological catalysts

1
FWhat type of substance is an enzyme? Write the word.
2
FWhat is a substance that speeds up a reaction and is not used up? Write the word.
3
FWhat is the name of the molecule that an enzyme acts on? Write the word.
4
FWhat is the part of an enzyme where the substrate fits? Write the words.
5
FWhat is the name of the model in which the substrate fits only one specific enzyme? Write the words.
6
FIs an enzyme used up in the reaction? Write yes or no.
7
FWhat are the products when amylase acts on starch? Write the word.
8
FWhat are the products when protease acts on proteins? Write the words.
9
FWhat are the products when lipase acts on fats? Write the words.
10
FEnzymes are biological catalysts. Is this statement true? Write yes or no.
11
PSAli says that each reaction changes the enzyme permanently. Is Ali right? Write yes or no.
12
PSWorking scientifically: a substrate does not fit the active site of an enzyme. Does the reaction happen? Write the words: not at all, or yes.
13
RExplain why an enzyme works on only one type of substrate. Write one sentence.
2.4

Temperature and pH

Year 8
8B2: the effect of temperature and pH on enzyme activity; denaturing; optimum conditions
Video lesson: Temperature and pHComing soon
Story. Omar tests how temperature affects the speed of starch breakdown. He uses the same amylase and the same starch in every test. “The hot tubes go faster,” he says, “until something happens at the top.” His teacher says, “You have found the optimum.”

Enzymes work fastest at a particular temperature, called the optimum. At low temperatures the molecules move slowly, so the reaction is slow. As the temperature rises, the enzyme and substrate collide more often, and the rate rises.

At high temperatures the enzyme's shape changes so that the active site no longer fits the substrate. The enzyme is denatured. Denaturing is not reversible. Each enzyme also has an optimum pH; a pH far from it slows the enzyme down.

Rate of starch breakdown at different temperatures10203040506005101520Temperature (degrees C)Rate (1000 / time)
The rate of starch breakdown rises to a peak near 40 degrees C, then falls as the enzyme denatures.

The table gives class data for starch breakdown at six temperatures. The rate is worked out as 1000 divided by the time.

Temperature (degrees C)Time for the iodine to stop turning blue-black (s)Rate (1000 / time)
102504
202005
3010010
405020
5010010
605002

The table gives the optimum pH of two enzymes.

EnzymeWhere it worksOptimum pH
pepsinstomachabout 2
salivary amylasemouthabout 7

Key ideas

  • Each enzyme works fastest at its optimum temperature.
  • Above the optimum, the enzyme denatures and the rate falls.
  • Denaturing is permanent: the active site loses its shape.
  • Each enzyme has its own optimum pH.
Worked example 2.4.1
Using the table, at which temperature is the rate highest? Write the number of degrees C.

Method. Find the largest rate.

  1. Read the rates. The rates are 4, 5, 10, 20, 10 and 2.
    highest is 20
  2. Name the temperature. The highest rate, 20, is at 40 degrees C.
    40
  3. Write the answer.
    40 degrees C

Check. Rates above and below 40 are lower. ✓

Worked example 2.4.2
Using the table, what is the rate at 30 degrees C? Write the number.

Method. Find the row for 30 degrees.

  1. Read. At 30 degrees C the time is 100 seconds.
    100 s
  2. Calculate. The rate is 1000 divided by 100.
    1000 / 100 = 10
  3. Write the answer.
    10

Check. 10 x 100 = 1000. ✓

Worked example 2.4.3
Which enzyme works best at about pH 2? Write the word.

Method. Find the optimum pH.

  1. Look. The optimum pH of pepsin is about 2, and it works in the stomach.
    about 2
  2. Name it. So the enzyme is pepsin.
    pepsin
  3. Write the answer.
    pepsin

Check. Salivary amylase works best at about pH 7. ✓

Watch out

Watch out: a high temperature does not make an enzyme work faster. Above the optimum, it denatures and the rate falls.

Exam tip

Exam tip. Say “denatured, the active site has changed shape, so the substrate no longer fits”.

Try it (with your teacher, or a grown-up at home). Ask your teacher to show the class the starch and iodine test, with a warm water bath and the same amount of starch and amylase in each tube. Note the time for the iodine to stop turning blue-black. Do not handle the hot water or the iodine yourself.

Exercise 2D  Temperature and pH

1
FWhat is the temperature at which an enzyme works fastest called? Write the word.
2
FAt a high temperature, the active site changes shape. What is this called? Write the word.
3
FIs denaturing reversible? Write yes or no.
4
FUsing the table, what is the rate at 20 degrees C? Write the number.
5
FUsing the table, at which temperature was the time the shortest? Write the number of degrees C.
6
FUsing the table, what is the difference between the rate at 40 degrees C and the rate at 50 degrees C? Write the number.
7
FWhich enzyme works in the stomach? Write the word.
8
FWhat is the optimum pH of pepsin, roughly? Write the number.
9
FWhat happens to an enzyme at a pH far from its optimum? Write one word: slows or speeds.
10
PSAli says the enzyme works best at 60 degrees C because it has the highest number in the table. Is Ali right? Write yes or no.
11
PSWorking scientifically: a pupil tests six temperatures, and each is repeated three times. How many tests are carried out? Write the number.
12
RExplain why a fair test of temperature must keep the pH the same. Write one sentence.
2.5

Osmosis and active transport

Year 8
8B3: diffusion revisited; osmosis through a partially permeable membrane; active transport against a concentration gradient
Video lesson: Osmosis and active transportComing soon
Story. Ruby puts potato cylinders into four different sugar solutions for one hour, then weighs each one. “The ones in plain water gained mass,” she says. “Why did the others lose mass?” Her teacher says, “Water moved out of them by osmosis.”

Diffusion is the net movement of particles from a high concentration to a low concentration, down a concentration gradient.

Osmosis is the diffusion of water through a partially permeable membrane, from a dilute solution (more water) to a concentrated solution (less water).

Active transport moves substances from a low concentration to a high concentration, against the concentration gradient. It needs energy from respiration. Plant root hair cells use it to absorb mineral ions from the soil.

membranedilute (more water)concentrated (less water)water
Osmosis: water moves through a partially permeable membrane from the dilute side to the concentrated side.
outside cellinside cellmoves against the gradientenergy from respiration is needed
Active transport: a substance moves against the concentration gradient, using energy.

The table gives the mass change of the potato cylinders after one hour.

Sugar solutionMass change (g)
0%: pure water+2.0
5% sugar+0.5
10% sugar-1.0
15% sugar-2.5

Key ideas

  • Diffusion: movement from high to low concentration down a gradient.
  • Osmosis: movement of water through a partially permeable membrane, from dilute to concentrated.
  • Active transport: movement against the gradient, using energy from respiration.
  • Osmosis needs no energy; active transport does.
Worked example 2.5.1
Which way does water move by osmosis: from dilute to concentrated, or from concentrated to dilute? Write the words.

Method. Recall the direction of osmosis.

  1. Recall. Water moves from the solution with more water to the solution with less water.
    more water
  2. Decide. So water moves from dilute to concentrated.
    dilute to concentrated
  3. Write the answer.
    from dilute to concentrated

Check. The concentrated solution takes water in. ✓

Worked example 2.5.2
Using the table, which sugar solution made the potato cylinder gain the most mass? Write the words.

Method. Find the largest positive mass change.

  1. Read. The largest gain is in the pure water, at +2.0 g.
    +2.0
  2. Name it. So the solution is pure water, 0%.
    0%: pure water
  3. Write the answer.
    0%: pure water

Check. Pure water is the most dilute. ✓

Worked example 2.5.3
Name a substance absorbed into root hair cells by active transport. Accept any one: mineral ions.

Method. Recall the example.

  1. Recall. Root hair cells absorb mineral ions from the soil by active transport.
    mineral ions
  2. Name it. So an example is mineral ions.
    mineral ions
  3. Write the answer.
    Accept any one: mineral ions.

Check. Mineral ions are in low concentration in the soil water. ✓

Watch out

Watch out: osmosis is the movement of water only. It is a special case of diffusion, through a membrane that lets water through but not all particles.

Exam tip

Exam tip. State the direction: osmosis from dilute to concentrated; active transport against the gradient, with energy.

Try it (with your teacher, or a grown-up at home). Look at the mass change table. With your teacher, explain why the cylinder in 10 percent sugar lost mass. Do not cut any potato yourself unless your teacher asks you to.

Exercise 2E  Osmosis and active transport

1
FWhat is the movement of particles from high to low concentration called? Write the word.
2
FWhat is the movement of water through a partially permeable membrane called? Write the word.
3
FIn osmosis, water moves from which solution to which? Write the words.
4
FDoes active transport move substances down or against a concentration gradient? Write the words.
5
FDoes active transport need energy? Write yes or no.
6
FWhere does active transport get its energy from? Write the word.
7
FName one substance that root hair cells absorb by active transport. Accept any one: mineral ions.
8
FUsing the table, what is the mass change of the potato in pure water? Write the number, with its sign.
9
FUsing the table, which solution gave the greatest loss of mass? Write the percentage.
10
FUsing the table, what is the mass change of the potato in 5 percent sugar? Write the number, with its sign.
11
PSAli says the potato lost mass in pure water. Is Ali right? Write yes or no.
12
PSRuby says diffusion and osmosis are the same process. Is Ruby right? Write yes or no.
13
RExplain why a root hair cell needs energy to absorb mineral ions from dilute soil water. Write one sentence.

Chapter summary

SkillMethod
OrganisationCell, tissue, organ, organ system. Example: stomach wall tissues make up the stomach.
DigestionMechanical (chewing, churning) makes food smaller; chemical (enzymes) changes molecules.
EnzymesProtein biological catalysts; lock-and-key active site; not used up.
Temperature and pHEach enzyme has an optimum; above the optimum it denatures, which cannot be reversed.
OsmosisWater moves through a partially permeable membrane from dilute to concentrated.
Active transportMovement against the gradient, needing energy from respiration.

Can you…?

  • I can put cells, tissues, organs and organ systems in order
  • I can name the organs of the digestive system and say what each does
  • I can explain mechanical and chemical digestion, and name the three main enzymes
  • I can use the lock-and-key model to explain how an enzyme works
  • I can read a temperature or pH table, and explain denaturing
  • I can explain osmosis and active transport, and say when each is needed

Key terms

tissue
a group of similar cells working together
organ
different tissues working together for one job
digestion
breaking down large insoluble food molecules into small soluble ones
enzyme
a protein that acts as a biological catalyst
substrate
the molecule an enzyme acts on
active site
the part of an enzyme where the substrate fits
denatured
an enzyme whose active site has changed shape, so it no longer works
osmosis
the movement of water through a partially permeable membrane, from dilute to concentrated
active transport
movement of substances against a concentration gradient, using energy from respiration

Exercise 2Z  Check-up

1
Name the organ system that includes the stomach and the liver. Write the words.
2
Which organ absorbs most of the digested food? Write the words.
3
Which enzyme breaks down starch into sugars? Write the word.
4
What is a substance that speeds up a reaction and is not used up? Write the word.
5
What is the part of an enzyme where the substrate fits? Write the words.
6
Using the table, what is the rate at 30 degrees C? Write the number.
7
What is the name of the process in which water moves through a partially permeable membrane? Write the word.
8
Which process needs energy from respiration to move substances against the gradient? Write the words.
9
Which enzyme works best in the stomach, at a pH of about 2? Write the word.
10
Explain why a high temperature stops an enzyme working. Write one sentence.

Total 14 marks. Allow about 14 minutes.

Exercise 2X  Practice test questions

1
FWhat is a group of similar cells working together called? Write the word.[1 mark]
2
FName the organ that makes bile. Write the word.[1 mark]
3
RDescribe the difference between mechanical and chemical digestion. Write one sentence.[2 marks]
4
FWhich enzyme breaks down fats? Write the word.[1 mark]
5
PSWhat is the optimum temperature of the enzyme in the table, given that the rate is highest at it? Write the number of degrees C, using the table of rates 4, 5, 10, 20, 10 and 2.[2 marks]
6
RExplain why an enzyme stops working when it denatures. Write one sentence.[2 marks]
7
FIn osmosis, water moves from the dilute solution to the concentrated solution. Is this statement true? Write yes or no.[1 mark]
8
FWhich process moves substances from a low concentration to a high concentration, using energy? Write the words.[1 mark]
9
FUsing the mass change table, which sugar solution gives a small gain of mass? Write the percentage.[1 mark]
10
RExplain why a fair test of enzyme activity keeps the amount of substrate the same. Write one sentence.[2 marks]

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